Revision of the World Species of Megaphragma Timberlake (Hymenoptera: Trichogrammatidae).

Megaphragma species are important models for basic organismal research, and many are potential biological control agents. We present the first extensive revision of species of the genus Megaphragma based on morphological and molecular data. Our revision includes all previously described species, 6 of which are synonymized, and 22 of which are described here as new. We also provide the first key to all species of the genus and reconstruct their phylogeny based on 28S and CO1 molecular markers. The following species are synonymized with M. longiciliatum Subba Rao: M. aligarhensis Yousuf and Shafee syn. nov.; M. amalphitanum Viggiani syn. nov.; M. decochaetum Lin syn. nov.; M. magniclava Yousuf and Shafee syn. nov.; M. shimalianum Hayat syn. nov.M. anomalifuniculi Yuan and Lou syn. nov. is synonymized with M. polychaetum Lin. The following species are described as new: M. antecessor Polaszek and Fusu sp. nov.; M. breviclavum Polaszek and Fusu sp. nov.; M. chienleei Polaszek and Fusu sp. nov.; M. cockerilli Polaszek and Fusu sp. nov.; M. digitatum Polaszek and Fusu sp. nov.; M. fanenitrakely Polaszek and Fusu sp. nov.; M. funiculatum Fusu, Polaszek, and Viggiani sp. nov.; M. giraulti Viggiani, Fusu, and Polaszek sp. nov.; M. hansoni Polaszek, Fusu, and Viggiani sp. nov.; M. kinuthiae Polaszek, Fusu, and Viggiani sp. nov.; M. liui Polaszek and Fusu sp. nov.; M. momookherjeeae Polaszek and Fusu sp. nov.; M. nowickii Polaszek, Fusu, and Viggiani sp. nov.; M. noyesi Polaszek and Fusu sp. nov.; M. pintoi Viggiani sp. nov.; M. polilovi Polaszek, Fusu, and Viggiani sp. nov.; M. rivelloi Viggiani sp. nov.; M. tamoi Polaszek, Fusu, and Viggiani sp. nov.; M. tridens Fusu, and Polaszek sp. nov.; M. uniclavum Polaszek and Fusu sp. nov.; M. vanlentereni Polaszek and Fusu sp. nov.; M. viggianii Fusu, Polaszek, and Polilov sp. nov.

An Integrative Study on Asphondylia spp. (Diptera: Cecidomyiidae), Causing Flower Galls on Lamiaceae, with Description, Phenology, and Associated Fungi of Two New Species.

An integrative study on some species of Asphondylia was carried out. Two species of gall midges from Italy, Asphondylia rivelloi sp. nov. and Asphondylia micromeriae sp. nov. (Diptera: Cecidomyiidae), causing flower galls respectively on Clinopodium vulgare and Micromeria graeca (Lamiaceae), are described and illustrated. The characteristics of each developmental stage and induced galls are described, which allowed the discrimination of these new species in the complex of Asphondylia developing on Lamiaceae plants. Molecular data based on sequencing both nuclear (ITS2 and 28S-D2) and mitochondrial (COI) genes are also provided in support of this discrimination. Phylogeny based on nuclear markers is consistent with the new species, whereas COI phylogeny suggests introgression occurring between the two species. However, these species can also be easily identified using a morphological approach. Phenology of host plants and gall midges are described, and some peculiar characteristics allow the complete and confident discrimination and revision of the treated species. Gall-associated fungi were identified as Botryosphaeria dothidea,Alternaria spp., and Cladosporium spp.

First Record of an Invasive Fruit Fly Belonging to Bactrocera dorsalis Complex (Diptera: Tephritidae) in Europe.

Emerging pests are increasingly threatening fruit orchard health across the Mediterranean area. Tephritidae, representing serious threats for Europe, are numerous, and the fruit flies Bactrocera zonata and those belonging to Bactrocera dorsalis complex are among the most alarming species. These species are highly polyphagous and B. zonata has already spread to some Mediterranean countries. Due to these ongoing threats, in the Campania Region (southern Italy), a survey with traps and infested fruits analysis was performed with the aim of detecting the presence of species of Bactrocera dorsalis complex. In two mixed fruit-trees fields, some adults belonging to a species of Bactrocera were captured in traps baited with the highly attractive male lure (methyl eugenol). They were distinguished from similar-looking Bactrocera spp. by morphological and molecular comparative analyses. Considering the existing morphological keys, specimens were tentatively identified as B. dorsalis but molecular characterization with COI split them into two clades. Some specimens were grouped with B. dorsalis similar to B. kandiensis and B. kandiensis and others in a clade including B. dorsalis and B. invadens (syn. B. dorsalis). ITS1 sequences instead confirmed morphological identification. The integrative approach allowed identifying all the specimens collected as belonging to the B. dorsalis complex. This finding represents the first field interception in Europe of a member of one of the most dangerous groups of fruit flies.

A New Gall Midge Species of Asphondylia (Diptera: Cecidomyiidae) Inducing Flower Galls on Clinopodium nepeta (Lamiaceae) From Europe, Its Phenology, and Associated Fungi.

A new gall midge, Asphondylia nepetae sp. n. Viggiani (Diptera: Cecidomyiidae), causing flower gall on Clinopodium nepeta (L.) Kuntze (Lamiaceae), is described from Europe. The morphological characteristics of adult, larvae, and pupa are described and illustrated. Molecular approach (by sequencing 28S-D2, ITS2, and COI) confirmed that A. nepetae is a distinct species. The development of the gall is always associated with the presence of the fungus Botryosphaeria dothidea (Moug.: Fr.) Ces. and De Not. (Botryosphaeriales: Botryosphaeriaceae). The new species can complete several generations per year, on the flowers of the same host plant and its adults emerge from late spring to autumn. Pupae overwinter inside peculiar flower galls in a state of quiescence. The impact of the pest is highly variable with a percentage of flowers infested that ranged between 3 and 57.5% in the sampled years. Insect mortality was, at least in part, due to parasitoids that attack the young stages of the midge. Among them, the dominant species was Sigmophora brevicornis (Panzer) (Chalcidoidea: Eulophidae).

A new species of Soikiella Nowicki (Hymenoptera: Trichogrammatidae) from Italy.

A new species of Soikiella Nowicki (Hymenoptera: Trichogrammatidae) that emerged mostly from galls induced by Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) on chestnut is described from Italy. The characterization of the new taxon, Soikiella italica Viggiani sp. n., was performed through a morpho-molecular approach. A key to the species of Soikiella is given. Emerging from the same samples were a few females of an unidentified species of Trichogramma Westwood that likely emerged from eggs of Lepidoptera associated with the galls. The true host of S. italica is also indicated to be the eggs of some insect other than D. kuriphilus that uses their galls as oviposition sites.

A new species of Poropoea Foerster from Africa (Hymenoptera, Chalcidoidea, Trichogrammatidae).

A new species of Poropoea Foerster (Hymenoptera, Chalcidoidea, Trichogrammatidae) was obtained from eggs of attelabid beetles (Coleoptera: Attelabidae) associated with Combretum sp. (Myrtales, Combretaceae). This species is described from Ogooué-Ivindo province in north-eastern Gabon. The new species is easily distinguished from the known members of the genus Poropoea by the following combination of characters: female antennal club unsegmented, premarginal vein of the fore wing with a nodular premarginal vein and the stigma of the stigmal vein black, the wing lacks the RS1 track; front and hind legs more robust than the middle leg and with coxa and femur markedly enlarged, and ovipositor exserted to one-third of the gaster length. Morphological features of this new species are discussed and illustrated. A key to females of Poropoea species lacking the Rs1 track in the fore wing has been constructed and is presented here.

Description of a new species of Oligosita Walker (Chalcidoidea: Trichogrammatidae), egg parasitoid of Balclutha brevis Lindberg (Homoptera: Cicadellidae) living on Pennisetum setaceum, from Italy.

A new species of Oligosita Walker (Chalcidoidea: Trichogrammatidae), O. balcluthae Viggiani et Laudonia n. sp., is described as a parasitoid of the eggs of Balclutha brevis Lindberg (Homoptera: Cicadellidae) associated with crimson fountain grass, Pennisetum setaceum (Poaceae) in Italy. Morphological features and biology of the new species are discussed and illustrated. The 28S-D2 and ITS2 regions were successfully amplified and sequenced.

On the misidentification of Anagrus ustulatus Haliday (Hymenoptera: Mymaridae).

The cosmopolitan genus Anagrus Haliday is one of the largest in Mymaridae (Hymenoptera: Chalcidoidea), and includes very common egg parasitoids, mostly of leafhoppers. The smallest species (body length: 0.4-0.6 mm) are found in the atomus species group. This group at present includes about 20 species worldwide. Anagrus atomus (Linnaeus) is among the most common species in the Palaearctic region. Other populations very similar in morphology and biology to this species are also widespread and associated with several leafhoppers infesting wild and cultivated plants (Matteucig & Viggiani 2008). Some specimens of these populations were identified as A. ustulatus Haliday by Chiappini (1989), who presumably followed Graham (1982).

Descripción de Lathromeroidea araguensis y registros de Adelograma pinto, nuevo género para Venezuela (Hymenoptera: Trichogrammatidae) / Description of Lathromeroidea araguensis and record of Adelogramma pinto genus to Venezuela (Hymenoptera: Trichogrammatidae)

Se describe Lathromeroidea araguensis sp. n., de Venezuela, y se registra un nuevo género de la familia Trichogrammatidae: Adelogramma Pinto 2006 para el país. Se mencionan los caracteres que la distinguen y se ilustran los caracteres diagnósticos. El holotipo y algunos paratipos serán preservados en la Colección Regional de Insectos de la Universidad Rómulo Gallegos, San Juan de los Morros, Venezuela. Otros paratipos serán preservados en la colección entomológica del Departamento de Entomología y Zoología Agraria Filippo Silvestri, Universidad de Nápoles Federico II, Portici, NA, Italia.